Objective: Developing new and adaptable cultivars suited to different environments is a primary goal for breeders. This study aimed to evaluate the genetic characteristics, including general and specific combining abilities, using a half diallel cross among nine bread wheat cultivars.
Method: Seeds of 36 F₁ hybrids derived from nine parents were planted in a randomized complete block design with three replications under normal and drought stress conditions. The parents and hybrids were evaluated based on morphological and agronomic traits such as plant height, days to maturity, spike length, peduncle length, thousand-seed weight, flag leaf area, and grain yield. Combining abilities and gene action were estimated using Griffing’s method 2 (B model) and analysis of variance following Jinks and Hayman's approach.
Results: Analysis with Griffing’s method 2 and Hayman-Jinks revealed significant effects of both general combining ability (GCA) and specific combining ability (SCA) across all traits and conditions, indicating the presence of both additive and non-additive genetic components. Parents Roshan, Shahpasand, and Azar2 exhibited high GCA for most traits under both conditions. Hayman’s variance analysis showed significant additive (a) and dominance (b) components for all traits in both environments. The significance of the b₂ component suggested an unequal distribution of dominant and recessive alleles. The b₃ component, indicative of SCA, was significant across traits. Heritability estimates ranged from 55% to 74% (broad-sense) and 14% to 61% (narrow-sense) in both conditions.
Conclusions: Traits such as plant height, spike length, peduncle length, and days to maturity under normal conditions, as well as spike length, peduncle length, thousand-seed weight, and flag leaf area under drought stress, can be effectively selected in early generations. For traits like seed weight, flag leaf area (normal conditions), plant height, days to maturity (drought stress), and grain yield in both conditions, selection should be delayed until advanced generations when traits are genetically fixed. Breeding methods such as pedigree, backcrossing, bulk, single seed descent, and doubled haploid techniques are recommended to accelerate genetic fixation.